Showing posts with label Phlebotomy. Show all posts
Showing posts with label Phlebotomy. Show all posts

The 10 Commandments of Phlebotomy


The 10 Commandments of Phlebotomy

By Dennis J. Ernst, MT(ASCP)

Blood specimen collection is one of the most underestimated procedures in health care. Because it looks deceptively simple, nurses, clinical nursing assistants, radiologic technologists, respiratory therapists, physicians and others are all being asked to "take a stab" at phlebotomy.
Knowledge of vein selection, the order of draw, test-specific handling, storage and transportation requirements, anatomy of the antecubital area, safety precautions, alternative sites and other factors make phlebotomy a highly technical procedure that takes months to learn and apply.
Like any other procedure, there are certain established and indisputable rules collectors should apply in order to consistently perform venipunctures cleanly, safely, successfully and with little or no discomfort to the patient. Some of these rules are so fundamental they could be collectively referred to as the commandments of phlebotomy.

Since 10 is a popular number for commandments, let's comply with tradition even though the list is admittedly much longer.

1 Thou shalt protect thyself from injury.
Today, drawing a sample of blood can potentially expose the health care worker to at least 20 communicable diseases. Most of them are life threatening, some of them cause incurable and fatal disease, but all of them may be preventable if health care workers use appropriate caution, technique and equipment.
It has been estimated that 1 million health care workers sustain accidental needlesticks every year. Thousands of these workers will contract some form of hepatitis; 50-60 of them will become seropositive for HIV. Hollow-bore needles, the kind used for collecting blood, account for 68.5 percent of all accidental needlesticks, and the use of winged infusion ("butterfly") sets account for 35 percent of accidental needlesticks. Even more alarming is that up to 92 percent of accidental needlestick injuries suffered by laboratory personnel go unreported. Avoiding the use of winged infusion sets and using gloves, needle disposal units and proper technique can significantly minimize the risk of injury.
2 Thou shalt identify thy patients.
This means referring to an identifying bracelet affixed to the patient or asking the patient to state his or her name. Because sedated or semi-conscious patients can respond affirmatively to any question, go beyond seeking an affirmation of their identity and request they state their name in full.
In the case of an emergency room patient whose full identity has not been established, a temporary identifier such as an assigned number is acceptable but should be amended when complete information is available. When positive identification is not possible by either of these methods, have the patient's nurse or other caregiver identify the patient and document the name of the person who verified the patient's identity for you. No other methods are acceptable.
3 Thou shalt puncture the skin at about a 15 degree angle.
Most textbooks agree that a 15-30 degree angle of insertion is optimal. This low angle of entry allows for a greater margin of error in judging the depth of penetration and greatly reduces the risk of passing through the vein and provoking underlying structures such as nerves, tendons and arteries. This is not to say that we all must start carrying around protractors to measure our angles, but inserting the needle at as low an angle as possible minimizes the risk to the patient and facilitates a successful puncture.
As an expert witness in cases involving injury to patients during venipuncture procedures, a majority of the nerve injuries I see involve an excessive angle of insertion. Injure a patient while puncturing at a steep angle and you will have a difficult time convincing the jury that you are immune from the standards as set forth in the literature.
4 Thou shalt glorify the medial vein.
Of the three veins in the antecubital area acceptable for venipuncture, the median cubital vein (in the middle) is the vein of choice for four reasons: 1) it's more stationary; 2) puncturing it is less painful to the patient; 3) it's usually closer to the surface of the skin; and 4) it isn't nestled among nerves or arteries.
When conducting your survey of the antecubital area, check both arms for the medial vein before considering one of the alternatives. If one is not prominent enough to instill confidence, default to the cephalic vein on the lateral or thumb side of the arm as a second choice. Keep the basilic vein (located on the medial or inside aspect of the antecubital area) as a last resort. The proximity of underlying nerves and the brachial artery make punctures in the area of this vein highly risky. Most permanent nerve injuries and arterial nicks I see result from misguided punctures into this vein. That is not to say the basilic vein should not be punctured. In many cases it is the prominent vein in the antecubital area.
However, when it is not visible and/or the initial puncture is unsuccessful, probing the area subjects the patient to the potential for excruciating pain and permanent injury more so than probing in the area of the cephalic or medial veins.
5 Thou shalt invert tubes containing anticoagulants immediately after collection.
A high percentage of specimens rejected by laboratories are due to clots in lavender- or blue-stoppered tubes. A quick inversion after collection prevents a second puncture. If not inverted immediately upon filling, invert the tubes as soon as possible after the puncture.
Drawing blood from a syringe requires extra consideration to prevent clotting. The moment blood enters the barrel of the syringe the clotting process begins. If the time it takes to fill the syringe and evacuate the specimen into the tubes exceeds 1 minute, significant clotting may take place. Not only will this make it difficult to evacuate the specimen through the needle and into the tubes, but if the clots are small enough to go undetected they can affect the accuracy of the results.
6 Thou shalt attempt to collect specimens only from an acceptable site.
Antecubital and hand veins are acceptable sites unless their use is precluded by intravenous infusions, injury or mastectomy. Any other site should be approached with great trepidation.
The anterior, or palm side, of the forearm is particularly susceptible to injury because of the close proximity of nerves and tendons to the surface of the skin and should not be considered.
Foot and ankle veins can be acceptable sites for venipunctures in some facilities and on some patients. However, puncturing these veins can lead to thrombophlebitis and clot formation in patients with coagulopathies or to tissue necrosis in diabetics. Therefore, before puncturing foot and ankle veins, make sure the facility does not have a policy against such punctures and that the physician approves of the site.
7 Thou shalt label specimens at the bedside.
There is no excuse for not completely labeling a specimen at the bedside. This means complete identification, not just temporary identifiers to remind you when you find time to label them completely later.
Patients have died as a result of mislabeled specimens. Case in point: At a small Midwestern hospital, a lab tech drew a specimen of blood to determine the blood type of a patient. She left the room without properly labeling the specimen, drew two more patients, then returned to the lab to type them all simultaneously. After an interruption, she returned to her workstation, misidentified the specimens and typed the patient incorrectly. The patient received incompatible blood and subsequently died.
Although this concept of complete and accurate specimen identification has been trumpeted loudly and clearly for decades, delayed labeling practices persist. On one ward at a large hospital, collectors scrawled patients' last names on the caps of the tubes to facilitate complete labeling at a later time. The bottom line is without exception: label the specimen completely at the bedside.
8 Thou shalt stretch the skin at the puncture site.
Pulling down on the skin from below the intended puncture site with the thumb of your free hand anchors the vein and stretches the skin through which the needle will pass. Anchoring the vein is particularly important when drawing from the cephalic or basilic veins. Stretching the skin is the single most effective way to minimize the pain of the puncture.
Routinely employing this technique has two potential bonuses: your rate of successful punctures goes up and your patients thank you for considering their suffering.
9 Thou shalt know when to quit.
Not everyone can draw blood from every patient. Even those who elevate phlebotomy to an art form can have difficulty from time to time. This is because there are veins intentionally placed in the antecubitals of the population at random for the sole purpose of keeping skillful collectors from becoming legends in their own minds. After two failed attempts, one should seriously consider sending in someone else. That's professionalism. It also may be the answer to your patient's prayers.
10 Thou shalt treat all patients as if they are family.
In a hospital, the only peace many patients experience is that which health care professionals bring them by their kind words, gentle technique and their smiles. Regardless of how you think your life led you to hold a position as a health care professional, consider yourself assigned by a higher authority because of the comfort you can offer to the sick and injured in your own unique and compassionate way. You haven't been employed; you've been ordained.
References

1. Jagger, J. (1998). Rates of needlestick injury caused by various devices in a university hospital. N Engl J Med, 319(5), 284-288.
2. Carlsen, W., & Holding, R. (1998, April 13). Epidemic rages caregivers: thousands die from diseases contracted through needle sticks. San Francisco Chronicle.
3. Pallatroni, L. (1998). Needlesticks: Who pays the price when costs are cut on safety? MLO, 30(7), 30-31, 34-36, 88.
4. Carlsen, W., & Holding, R. (1998, April 14). High profits--at what cost? San Francisco Chronicle.
5. Jagger, J. Risky procedure, risky devices, risky job. Advances in Exposure Prevention, 1(1).
6. Garza, D., & Becan-McBride, K. (1999). Phlebotomy handbook: Blood collection essentials. Norwalk, CT: Appleton & Lange.
Dennis J. Ernst is director of the Center for Phlebotomy Education and also teaches phlebotomy at the University of Louisville School of Allied Health Sciences. Readers may purchase the author's "Ten Commandments of Phlebotmy" poster through the Center for Phlebotomy Education. The 16x20 four-color graphic can be viewed and ordered at www.phlebotomy.com/poster.htm.

Phlebotomy Procedures Materials Required


The following equipment should be assembled before proceeding with the venipuncture procedure:

Blood Drawing Site: The blood drawing site should provide a chair with a wide, flat, clean surface on the arms, or the patient may be lying in bed. The area should be wide enough for the patient's arm to rest comfortably. The patient's elbow should be supported so that it remains straight.
Some outpatients may prefer lying down, in which case an examination table may be used.

Blood Collection Safety Needles: "Puncture-guard" needles by BioPlexus are available in sizes 21 gauge and 22 gauge, 1 inch to 1.5 inches. We also have available safety "winged" sets in sizes of 21 ga, 23ga, 25 ga and 27 ga.

Plastic Tube/Needle Holders: The plastic holders are used to hold the needle on one end, and the vacuum tube for collection on the other. Regular, and blood culture sizes are available.

Blood Collection Vacuum Tubes: The vacuum tubes are designed to draw a predetermined volume of blood. Regular, pediatric tubes, and microtainers are available in a variety of sizes. Tubes with different additives are used for collecting blood specimens for specific types of tests. The color of the rubber stopper capping the tube is used to identify these additives, which, in turn, dictate the function of the tube.

Tourniquets: Tourniquets are used to help distend the veins for ease in venipuncture. Easy to use Velcro lined tourniquets are available in adult and pediatric sizes. Most commonly used type is 1"x16" non-latex band.

Antiseptics: Individually packaged 70% isopropyl alcohol wipes may be used to clean the venipuncture site for most specimens. Cepti Seal Blood Culture Prep kits must be used when collecting specimens for blood cultures. (See "Collection of Blood Cultures" section for further explanation).

Gauze: 2 x 2 inch gauze squares can be folded and taped to the puncture site to control the bleeding after the blood specimen is collected.

Sharps Disposal Container: An OSHA acceptable, puncture proof, container marked "Biohazardous" with a top for unscrewing needles must be used for needle disposal, or for partially filled capillary tubes.

Ammonia Inhalants: Ammonia inhalants may be used to revive patients who faint or become dizzy.

Cold Compress: Cold compresses may be used to revive patients who faint or become dizzy.
Disinfectant: A plastic wash bottle with an ASCEND solution should be available for cleaning up small blood spills.

Adhesive Labels: 1.0 x 2.5-inch adhesive labels and a permanent marking pen should be available for labeling the specimens.

Non-Powdered Gloves: Latex gloves may be found in various sizes. Also available are various types of non-latex gloves.

Post Blood Collection Hematoma Care Instructions


What is a hematoma?
A hematoma is a swollen or raised area at the venipuncture site resulting from the leakage of blood into the tissues.
What will happen to the hematoma?
In the next few days, the blood will be absorbed by the body. The blood will surface to the skin causing a bruised appearance. As the bruise is healing, it will turn a yellowish-green in color and then gradually fade.
What can I do?
Below are a few steps you can take to help the healing process and/or make your arm feel more comfortable:
1. Leave the pressure dressing on for at least 8 hours.
2. Do not take aspirin or ibuprofen for 72 hours
3. Avoid lifting heavy objects with the arm
4. Apply ice packs, wrapped in a cloth, to the affected site for approximately 20 minutes one or more times during the first 24 hours following the formation of the bruise or hematoma.
5. You may apply warm, moist compresses to the site for 20 minutes one or more time during the second 24 hours after the collection.
6. If you notice any of the following complications notify your doctor immediately and report the problem to us.
• Discoloration of the hand
• Additional swelling
• Generalized pain or discomfort of the arm
• Throbbing of the arm
• Numbness in the arm

If you have any questions or concerns, please contact your medical provider

Cara pengambilan darah (pada anak-anak)


(milis-nakita] Tonang D Ardyanto
Sering muncul pertanyaan: kok ambil darahnya lain-lain, ada yang di jari, ada yang di lengan, kan sakit buat anak-anak.
Begini ceritanya.
Penentuan posisi pengambilan sampel darah, terutama ditentukan oleh jenis pemeriksaan yang akan dilakukan. Pertimbangan untuk sesedikit mungkin menimbulkan trauma tentu tetap penting.

Pengambilan melalui ujung jari, disebut "darah kapiler". Sedangkan pengambilan di siku-dalam disebut "darah vena". Selain siku-dalam, bisa juga di tempat lain, hanya siku-dalam lebih disukai karena lebih menguntungkan.

Darah kapiler bisa dipakai untuk tujuan pemeriksaan hematologi (darah) rutin : penyaring dasar anemia (Hb, sel darah merah, hematokrit), penghitungan sel darah, golongan darah. Memang ada kekurangan, tapi dianggap bisa diabaikan untuk hematologi rutin ini.

Bagaimana dengan tes gula darah yang hanya pakai tetesan itu? Ini bagian dari konsep PoCT (Point of Care Test). Pengukuran kadar gula darah dengan darah vena itu disebut wet-chemistry sedang yang kita lihat portable itu "dry-chemistry".

Apa boleh dipakai? Dipakai boleh, dijadingan pegangan jangan. Artinya, pemakaian di rumah secara berkala silakan, tetapi cross-check tetap harus ke laboratorium. Faktor "kondisi darah" maupun teknik pemeriksaan dengan dry-chemistry sangat mempengaruhi hasil. Karena itu, secara berkala 1 minggu sekali periksa di rumah silakan, tetapi pemeriksaan rutin tetap ke
laboratorium.

Sedangkan untuk keperluan pemeriksaan yang lebih lengkap, perlu darah vena :
penyaringan anemia spesifik (misalnya menyaring anemia defisiensi zat besi), untuk gambaran darah tepi (blood-smear), maupun untuk keperluan pemeriksaan kimiawi darah (kadar bilirubin, kadar gula, kadar lemak, kadar protein, elektrolit darah, dsb).

Soal cara pengambilan sampel, kalau dilihat dari sisi pemeriksaan saja, tentu darah vena lebih baik. Tetapi dari sisi kenyamanan, tentu kalau masih bisa dengan darah kapiler, akan lebih nyaman bagi pasien.

Ilmu pengambilan darah ini disebut phlebotomy (sebenarnya lebih luas daripada sekedar pengambilan sampel darah atau pengambilan darah di PMI, ini sederhananya saja). Ada perangkat persyaratan dan pelatihan standar untuk menjalankan tugas sebagai phlebotomist.

Sebagaimana terjadi di kalangan profesi perawat, dulunya para petugas pengambil sampel darah ini juga hanya berdasarkan pengalaman lapangan bertahun-tahun. Tetapi sekarang sudah ada standarissasi dan masuk dalam parameter baku-mutu laboratorium.

Pegangan nomor 1 seorang phlebotomis adalah : YAKIN. Menentukan lokasi pengambilan, lokasi pembuluh darah, ciri-ciri pasien seperti apa yang kemungkinan ada hambatan, yang ada risiko pembekuannya lambat, ada ilmunya. Begitu seorang phlebotomis tidak yakin, maka dia harus langsung mundur dan digantikan yang lain. Begitu juga, kalau 2 kali mencoba gagal, akan
digantikan yang lain.

Beberapa ciri yang lebih mungkin ada hambatan pengambilan :
1. Anak gemuk,sehingga tidak mudah menentukan lokasi pembuluh darah
2. Anak dengan pembuluh darah kecil, biasanya anak perempuan lebih kecil
ukurannya, sehingga lebih sulit diambil.
3. Anak dengan pola posisi pembuluh darah yang berbeda. Meski ada pola umum,
ada pula yang polanya berbeda, sehingga phlebotomis harus mencari lebih
lama.

Harap dipahami, tidak pula rasional kalau kita lantas mudah memarahi mereka.
Membuat phlebotomis gelisah karena kita marah, hanya akan memperbesar risiko kegagalan mereka menjalankan tugas.

Yang lebih rasional :
1. Pastikan petugas phlebotomis sudah tahu persis dan mengecek identitas anak kita. Jangan sampai terjadi salah identitas. Pengalaman akhir-akhir ini makin banyak kesamaan nama yang "indah-indah". Juga - maaf bukan bermaksud SARA - yang menggunakan nama Tionghoa/Suku tertentu, perlu sekali dipastikan karena tidak semua petugas memahami konsep "family-name" sehingga bisa
terjadi keliru. Lebih baik kita ikut susah-payah sedikit memastikan hal ini, karena bisa terjadi kesalahan yang murni tanpa kesengajaan petugas, selain karena benar-benar tidak tahu.
2. Kalau anak kita pernah diambil sampel darahnya, sampaikan pengalaman itu.
Apakah saat itu ada hambatan, di lengan yang mana, kapan terakhir diambil, bagaimana reaksi setelah diambil. Ini sangat berguna bagi phlebotomis.
3. Tanyakan "berapa banyak yang akan diambil, cukup sekali dengan satu alat, atau harus dua kali?" (untuk pemeriksaan tertentu, memang harus ada beda perlakuan sampel darah, sehingga bisa saja diambil secara terpisah).
4. Setelah itu tanyakan "menurut Anda anak saya ini akan sulit atau tidak diambil sampel darahnya?"

Prosedur pengecekan identitas, pertanyaan rentang riwayat pengambilan sampel sebelumnya, penjelasan tentang berapa volume darah dan teknik yang akan diambil, sudah menjadi prosedur baku untuk dijelaskan. Dengan kita aktif bertanya dan menjelaskan, akan makin kecil risiko adanya data yang terlewatkan.

Pertanyaan dari pasien juga akan memberi kesempatan kepada dua pihak untuk saling mengukur keyakinan diri. Kalau nanti si petugas menyatakan sulit, baru kita teruskan "Anda yakin mengambil sampel darah anak saya?". Dengan tahapan seperti ini, si petugas tidak akan mengedepankan emosi - yang tentu saja sebenarnya tidak diperbolehkan apapun alasannya.

Begitu juga kalau memang si petugas yakin, beri dia dukungan agar makin yakin. Kalau berhasil dengan mulus, sampaikan terima kasih. Kalaupun kemudian memang gagal, sampaikan "apa tidak sebaiknya Anda minta diganti yang lain agar lebih yakin?". Dengan langkah-langkah seperti ini, bisa terhindarkan kekakuan hubungan yang tidak diperlukan.

Sekarang, alat pengambilan sampel darah sudah makin maju, tidak lagi menggunakan tabung suntik seperti dulu. Ada tabung khusus yang bersifat "vacuum" (bertekanan negatif) sehingga rasa sakit lebih ringan sekaligus memperkecil risiko darah-beku saat baru saja diambil. Tetapi, ada saatnya pula pengambilan tetap menggunakan jarum biasa, karena keperluan pemeriksaan
tertentu (karena ada beda perlakuan terhadap sampel darah, tidak sama dengan sampel darah untuk pemeriksaan darah secara umum).

Pengambilan sampel darah relatif lebih sulit pada bayi, yang makin muda. Perlu teknik tinggi dan pengalaman lapangan lama. Tempatnya sering harus mencari-cari yang paling memungkinkan. Paling disukai tetap di siku-dalam, tetapi bisa juga di kaki.

Kalau anak kita dirawat di RS, ada lagi prosedur pengambilan darah yang memang secara teknis lebih sulit, yaitu untuk pemeriksaan Blood-Gas Analysis (BGA : analisa gas darah). Yang diperlukan adalah "darah arteri" bukan darah vena. Biasanya diambil dari arteri femoralis (di bagian pangkal paha). Warna darahnya(lebih cerah) beda dengan darah vena (lebih gelap). Setelah diambil sesegera mungkin dihindari dari kontak dengan udara dan diperiksa secepat-cepatnya.

Memang, sekolah phlebotomis "hanya" bisa mengajarkan ilmu, teori, latihan pada manequin dan sedikit latihan pada pasien. Keterampilan hanya bisa diperoleh dari pengalaman.
Ada kemungkinan pengambilan sampel darah pada Anda atau anak Anda tidak bisa sekali berhasil. Namun, dari pengalaman seperti itulah phlebotomis akan makin terampil.

Tonang
dokter umum (bukan SpPK).
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